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2.6.FermentationexperimentsBatchcultureexperimentswerecarriedoutinpH-controlled500mlfleakerswithaworkingvolumeof350mlundersemianaerobicconditionsessentiallyasdescribedpreviously[15].Ricehullhydrolyzatewasusedassubstrate.Themediumwaspreparedbydissolving10gtryptoneand5g...顯示更多2.6.FermentationexperimentsBatchcultureexperimentswerecarriedoutinpH-controlled500mlfleakerswithaworkingvolumeof350mlundersemianaerobicconditionsessentiallyasdescribedpreviously[15].Ricehullhydrolyzatewasusedassubstrate.Themediumwaspreparedbydissolving10gtryptoneand5gyeastextractinthedilutedhydrolyzate(perl)andautoclavingat121?Cfor15min.A4MKOHsolutionwasusedforpHcontrol.Sampleswerewithdrawnperiodicallytodeterminecelldensity,residualsugars,andethanolandstoredat?20?Cpriortoanalysis.BaseconsumptionandpHwerealsorecorded.ForSHFexperiments,thefermentationwasperformedatpH6.5,35?Cand130rpmusingtheliquidportionoftheenzymaticallysaccharifiedricehullhydrolyzate.ForSSFexperiments,2lfermenters(BiostatB,B.BraunBiotechInternational,Allentown,PA)withworkingvolumesof1.5lwereusedatpH6.0and35?Cattheagitationrateof150rpm.ThealkalineperoxidepretreatedwholericehullhydrolyzatewasaddedtothefermenterassubstrateafterdilutingthreetimesandadjustingthepHto6.0withconcentratedHClbeforeaddingenzymesandinoculum.Inoculumsizewas5%(v/v)inbothcases.
意思： 2.6. 發酵實驗批文化實驗如所描述早先根本上被執行了在酸鹼度受控500 機器語言fleakers 以350 機器語言的工作容量在semianaerobic 情況下[ 15 ] 。米船身hydrolyzate 被使用了作為基體。媒介由溶化準備了10 g tryptone 和5 g 酵母抽提物在被稀釋的hydrolyzate (每l) 和真空加熱在121?C 15 分鐘。4M KOH solutionwas 被使用為酸鹼度控制。樣品階段性地被撤出確定細胞密度、殘餘的糖, 和對氨基苯甲酸二和被存放了在?20?C 在分析之前。基本的消耗量和酸鹼度並且被記錄了。為SHF 實驗, 發酵進行了在酸鹼度6.5, 35?C 和130 轉每分鐘使用enzymatically 糖化的米船身hydrolyzate 的液體部份。為SSF 實驗, 2 升發酵桶(Biostat B, B. Braun Biotech 國際, Allentown, PA) 以1.5 升的工作容量被使用了在酸鹼度6.0 和35?C 以150 轉每分鐘的鼓動率。鹼性過氧化物被預處理的整體米船身hydrolyzate 增加了來發酵桶作為基體在稀釋三次和調整酸鹼度以後到6.0 與被集中的HCl 在增加酵素和接種物之前。接種物大小是5% (v/v) 在兩個案件。
hydrogenchloride
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